Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted osmotic shock method

H. Hadiyanto, - and Nais Pinta Adetya, - (2018) Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted osmotic shock method. Food Science and Biotechnology, 27 (5). pp. 1361-1368. ISSN 2092-6456

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Abstract

In this study, we optimized the process for extracting lipids and proteins from wet biomasses of Spirulina sp. using a 4-kHz ultrasonic osmotic shock method with ultrasound enhancement at a constant frequency of 40 kHz. Optimization was conducted using a response surface methodology (RSM) at an osmotic NaCl concentration of 10–30%, solvent:biomass ratio of 5–15 v/ w, and extraction times of 20–50 min. The present osmotic shock method with ultrasound irradiation increased lipid yields to 6.65% in the presence of 11.9% NaCl, a solvent:biomass ratio of 12:1 v/w, and a 22-min extraction time, and protein yields to 43.96% with 15.12% NaCl, a solvent:biomass ratio of 10:1 v/w, and a 30-min extraction time.

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